Mysore Analytical Services

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Module 3 - HPLC

Overview

Training Module 3: HPLC Training

This 15-day focused training module is designed to build practical proficiency in High Performance Liquid Chromatography (HPLC). It covers essential aspects such as operation, calibration, troubleshooting, regulatory compliance, and lab best practices—making it ideal for entry-level analysts, students, and quality control professionals.

Duration: 15 Days | Mode: Theoretical & Practical (Hands-On)

Core Training Areas

HPLC Operation, Calibration & Troubleshooting

Participants will learn:

  • Instrument setup and operational workflow

  • Calibration procedures for accuracy and precision

  • Troubleshooting common issues in HPLC systems

  • Real-time sample analysis and result documentation

The program includes essential GLP skills to ensure regulatory compliance and analytical integrity:

  • SOP (Standard Operating Procedure) preparation
  • Specification & Method of Analysis drafting
  • Volumetric solutions & reagent preparation
  • Standard & sample preparation techniques
  • Pipetting skills
  • Management of standards (Primary Reference, Working, and Impurity Standards)
  • QMS (Quality Management System) and lab safety protocols

SANTE Guidelines

Residue analysis and method criteria (for food/pharma)

ICH Guidelines

Analytical validation, documentation standards

  • Technical presentation skills
  • Mock interviews based on industry requirements
  • HPLC system (complete operational and practical exposure)

Outcome

Upon completion, participants will have job-ready skills in HPLC operation and documentation, be confident in lab procedures, and prepared for entry into QC, QA, or ADL roles in food, pharma, or chemical industries.